期刊
PROTEOMICS
卷 10, 期 12, 页码 2303-2319出版社
WILEY
DOI: 10.1002/pmic.200900493
关键词
2-D DIGE; Fusarium graminearum Delta Tri5 infection; Plant proteomics; Wheat development; Xylanase inhibitor families
资金
- Fonds voor Wetenschappelijk Onderzoek-Vlaanderen (F.W.O., Brussels, Belgium)
- Instituut voor de aanmoediging van Innovatie door Wetenschap en Technologie in Vlaanderen (I.W.T., Brussels, Belgium)
- K.U.Leuven Research Fund [GOA/03/10]
- Methusalem programme Food for the Future at the K.U.Leuven
Wheat contains three different classes of proteinaceous xylanase inhibitors (XIs), i.e. Triticum aestivum xylanase inhibitors (TAXIs) xylanase-inhibiting proteins (XIPs), and thaumatin-like xylanase inhibitors (TLXIs) which are believed to act as a defensive barrier against phytopathogenic attack. In the absence of relevant data in wheat kernels, we here examined the response of the different members of the XI protein population to infection with a Delta Tri5 mutant of Fusarium graminearum, the wild type of which is one of the most important wheat ear pathogens, in early developing wheat grain. Wheat ears were inoculated at anthesis, analyzed using 2-D DICE and multivariate analysis at 5, 15, and 25 days post anthesis (DPA), and compared with control samples. Distinct abundance patterns could be distinguished for different XI forms in response to infection with F. graminearum Delta Tri5. Some (iso)forms were up-regulated, whereas others were down-regulated. This pathogen-specific regulation of proteins was mostly visible at five DPA and levelled off in the samples situated further from the inoculation point. Furthermore, it was shown that most identified TAXI- and XIP-type XI (iso)forms significantly increased in abundance from the milky (15 DPA) to the soft dough stages (25 DPA) on a per kernel basis, although the extent of increase differed greatly. Non-glycosylated XIP forms increased more strongly than their glycosylated counterparts.
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