期刊
PROTEOMICS
卷 10, 期 21, 页码 3922-3927出版社
WILEY
DOI: 10.1002/pmic.201000219
关键词
Antibody immobilization; Biochip; Biomarker; Mass spectrometry; Technology
资金
- National Institutes of Health [R03 CA10833]
A novel approach to immunoaffinity MS is described wherein antibodies are appended to a patterned gold Biochip surface. The Biochip surface is patterned with an array of concentric immunocapture zones composed of highly hydrophilic central zones surrounded by moderately hydrophilic zones that reside on a non-wetting background, with protein attachment via electrochemically cleavable linkers. After linker cleavage, matrix application forms a discrete spot suitable for MALDI-TOF-MS. Use of the Biochip to purify transthyretin from human serum allowed a distinct resolution of four disulfide conjugates and one truncated form isoforms with good mass resolution and sensitivity.
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