期刊
PROTEOMICS
卷 10, 期 14, 页码 2728-2732出版社
WILEY
DOI: 10.1002/pmic.201000039
关键词
1-D gel electrophoresis; 2-D chromatography; Cell biology; Embryonic stem cell; Isoforms; Shotgun proteomics
资金
- NIH, National Institute on Aging
- NIH [K99-L094708-01]
- NHLBI Proteomics Innovation [N01-HV-28180, NIH-R01-HL085434]
- AHA [09GRNT2500002]
The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, Rl. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection of an isoform-specific peptide. When compared with two previously published proteomic studies that used the same cell line, the current study reveals 612 new proteins.
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