期刊
PROTEOMICS
卷 10, 期 3, 页码 458-469出版社
WILEY
DOI: 10.1002/pmic.200900307
关键词
Azoarcus; Endophytic bacterium; Microbiology; Proteome reference map; Response regulator PilR
资金
- University of Bremen [ZF 02/114/05]
- German Federal Ministry of Education and Research (BMBF, GenoMik network [0313105]
- Deutsche Forschungsgemeinschaft (DGF) [756-SFB395]
The proteome of the grass endophyte Azoarcus sp. strain BH72 was analyzed by a combination of gel-based methods by means of 2-DE and MS and a gel-free approach via LC-MS/MS. Among the identified 785 proteins, synthesis of around 100 conserved hypothetical proteins could be confirmed. Membrane proteins were detected at a higher rate in the gel-free than in the gel-based approach. The abundance of proteins in the constructed proteome reference map was analyzed and the 30 most abundant proteins were determined. The reference map was then used as a starting point to characterize the regulon under control of the response regulator PilR. PilR is part of the two-component regulatory system PilSR controlling type IV pilin gene (pilAB) expression in strain BH72, which was strongly decreased (19.7-fold) in the pilR-mutant. Changes of protein composition in the wild type and the regulatory mutant were compared by the gel-based and gel-free analyses. Proteins responsible for amino acid and energy metabolism, chaperones as well as proteins that are involved in iron metabolism and iron storage were present in a pilR-mutant at different levels than in the wild-type strain. Levels of the transcriptional regulator Flp were also dependent on PilR, indicating that PilR might be part of a hierarchical regulatory cascade.
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