期刊
PROTEOMICS
卷 9, 期 21, 页码 4970-4973出版社
WILEY
DOI: 10.1002/pmic.200900251
关键词
Agricultural soil; Glomalin; Protein extraction; Soil proteomics; Technology
资金
- Program for Science&Technology Innovation Talents in Universities of Henan Province [2008HASTIT005]
- Innovation Scientists and Technicians Troop Construction Projects of Henan Province, P. R. China [09410051003]
Soil contains low amounts of protein but high amounts of interfering substances. Current extraction methods for soil protein cannot produce high-quality samples suitable for proteomic analysis. To resolve the problem, we devised a sequential extraction method, through sequentially extracting soil in citrate and SIDS buffers, followed by phenol extraction. The method allows for obtaining applicable 1-D and 2-D protein profiles with various agricultural soils and detecting glomalin-related soil protein. The method may be a valuable tool for soil proteomics.
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