期刊
PROTEOMICS
卷 9, 期 5, 页码 1407-1415出版社
WILEY
DOI: 10.1002/pmic.200800394
关键词
2-DE; Aspergillus fumigatus; Data warehouse; Mitochondrial proteome; Reference map
资金
- Deutsche Forschungsgemeinschaft (DFG)
The filamentous fungus Aspergillus fumigatus has become the most important airborne fungal pathogen causing life-threatening infections in immunosuppressed patients. We established a 2-D reference map for A. fumigatus. Using MALDI-TOF-MS/MS, we identified 381 spots representing 334 proteins. Proteins involved in cellular metabolism, protein synthesis, transport processes and cell cycle were most abundant. Furthermore, we established a protocol for the isolation of mitochondria of A. fumigatus and developed a mitochondrial proteome reference map. 147 proteins represented by 234 spots were identified.
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