期刊
PROTEOMICS
卷 9, 期 11, 页码 2995-3010出版社
WILEY
DOI: 10.1002/pmic.200800016
关键词
Candida; 2-D PAGE; Differential expression; Inflammation; Macrophages
资金
- Comision Inter-ministerial de Ciencia y Tecnologia (CYCIT Spain) [BIO 01989-2006]
- Comunidad Autonoma de Madrid [S-SAL-0246-2006]
- Miniterio de Sanidad y Consumo
- Instituto de Salud Carlos III - FEDER
- Spanish Network for the Research in Infectious Diseases [REIPI RD06/0008]
- Ministerio de Educacion
Murine macrophages (RAW 264.7) were allowed to interact with heat-inactivated cells of Candida albicans SC5314 during 45 min. The proteomic response of the macrophages was then analyzed using 2-D gel electrophoresis. Many proteins having differential expression with respect to control macrophages were identified, and their functions were related to important processes, such as cytoskeletal organization, signal transduction, metabolism, protein biosynthesis, stress response and protein fate. Several of these proteins have been described as being involved in the process of inflammation, such as Erp29, Hspa9a, AnxaI, Ran GTPase, P4hb, Clic1 and Psma1. The analysis of the consequences of their variation unravels an overall anti-inflammatory response of macrophages during the interaction with heat-inactivated cells. This result was corroborated by the measurement of TNF-alpha and of ERK1/2 phosphorylation levels. This anti-inflammatory effect was contrary to the one observed with live C. albicans cells, which induced higher TNF-alpha secretion and higher ERK1/2 phosphorylation levels with respect to control macrophages.
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