期刊
PROTEOMICS
卷 9, 期 5, 页码 1197-1206出版社
WILEY
DOI: 10.1002/pmic.200800333
关键词
Chronic lymphocytic leukemia; Histone; RPLC-MS; Variant
资金
- Ohio State University
- Camille and Henry Dreyfus Foundation
- V-Foundation
- National Institutes of Health [CA101956, CA107106, RR023647, CA102031, CA134232]
- Leukemia and Lymphoma Society
- Leukemia 9 Lymphoma Society
- D. Warren Brown foundation
The in vitro evaluation of histones and their PTMs has drawn substantial interest in the development of epigenetic therapies. The differential expression of histone isoforms may serve as a potential marker in the classification of diseases affected by chromatin abnormalities. In this study, protein profiling by LC and NIS was used to explore differences in histone composition in primary chronic lymphocytic leukemia (CLL) cells. Extensive method validations were performed to determine the experimental variances that would impact histone relative abundance. The resulting data demonstrated that the proposed methodology was suitable for the analysis of histone profiles. In 4 normal individuals and 40 CLL patients, a significant decrease in the relative abundance of histone H2A variants (H2AFL and H2AFA/M*) was observed in primary CLL cells as compared to normal B cells. Protein identifies were deter-mined using high mass accuracy MS and shotgun proteomics.
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