期刊
PROTEOMICS
卷 9, 期 13, 页码 3463-3473出版社
WILEY
DOI: 10.1002/pmic.200800722
关键词
Carbonate extraction; DnaJC25; ER; MS; Two-dimensional gel electrophoresis
资金
- Deutsche Forschungsgemeinschaft [SFB 530, FZT-82]
- Saarland Ministry for Economic Affairs and Science (LFFP)
- Leibniz Association
The rough ER (rER) plays a central role in the biogenesis of most extracellular and many organellar proteins in eukaryotic cells. Cells that are specialized in protein secretion, such as pancreatic cells, are particularly rich in rER. In the process of cell homogenization, the rER is converted into ribosome-studded vesicles, the so-called rough microsomes. Here we report on a membrane proteomic analysis of canine pancreatic rough microsomes. Special emphasis was placed on components involved in the various aspects of protein biogenesis, such as protein transport, protein folding, protein modification, and protein degradation. Our results indicate that the Hsp70-chaperone network that is present in the pancreatic ER is even more complex than previously thought, and suggest that the pancreatic rER has a significant capacity for protein degradation.
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