期刊
PROTEOMICS
卷 8, 期 22, 页码 4633-4636出版社
WILEY
DOI: 10.1002/pmic.200800326
关键词
Enterotoxin; Food poisoning; Mass spectrometry; Protein standard absolute quantification; Quantification
资金
- Sanofi-Aventis France and Bayer Pharma
- French Agency for Research [ANR-06-PNRA-029]
Staphylococcal enterotoxins are major causing agents of food-borne diseases. Their detection in food remnants for risk assessment or food poisoning outbreaks investigation suffers from a lack in comprehensive immunological tools. In this study, we demonstrate that the combination of immunocapture and Protein Standard Absolute Quantification (PSAQ) strategy, which uses isotope-labeled enterotoxins as internal standards for MS-based analysis, is powerful to specifically identify and quantify these contaminating agents in food matrices. This approach is believed to significantly improve the elucidation of staphylococcal food poisoning outbreaks.
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