期刊
PROTEOMICS
卷 8, 期 8, 页码 1539-1550出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200701044
关键词
C-terminal peptide; isolation; mass spectrometry; post-translational modification; sequencing
A simple and efficient method for C-terminal sequencing of proteins has long been pursued because it would provide substantial information for identifying the covalent structure, including post-translational modifications. However, there are still significant impediments to both direct sequencing from C termini of proteins and specific isolation of C-terminal peptides from proteins. We describe here a highly successful, de novo C-terminal sequencing method of proteins by employing succinimidyloxycarbonylmethyl tris (2,4,6-trimethoxyphenyl) phosphonium bromide and mass spectrometry.
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