期刊
PROTEOMICS
卷 8, 期 1, 页码 32-36出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200700707
关键词
alignment; data quality; LC-MS; mass spectrometry; quantitative proteomics
Comparative LC-MS is a powerful method for detailed quantitative comparison of complex protein mixtures. Dedicated software is required for detection, matching, and alignment of peaks in multiple LC-MS datasets. However, retention time shifts, saturation effects, limitations of experimental accuracy, and possible occurrence of split peaks make it difficult for software to perfectly match all chromatograms. We describe a procedure to assess the above problems and show that dataset quality can be enhanced with the aid of cluster analysis.
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