期刊
PROTEIN SCIENCE
卷 20, 期 12, 页码 2095-2101出版社
WILEY-BLACKWELL
DOI: 10.1002/pro.749
关键词
DAUDA; fluorescent fatty acid analogue; crystal structure; human serum albumin
资金
- Natural Science Foundation of China [30800181, 31161130356]
- Natural Science Foundation of Fujian [2009\0029]
- Ministry of Science of Technology [2007CB914304]
11-(Dansylamino) undecanoic acid (DAUDA) is a dansyl-type fluorophore and has widely used as a probe to determine the binding site for human serum albumin (HSA). Here, we reported that structure of HSA-Myristate-DAUDA ternary complex and identified clearly the presence of two DAUDA molecules at fatty acid (FA) binding site 6 and 7 of HSA, thus showing these two sites are weak FA binding sites. This result also show that DAUDA is an appropriate probe for FA site 6 and 7 on HSA as previous studied, but not a good probe of FA binding site 1 that is likely bilirubin binding site on HSA.
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