期刊
PROTEIN SCIENCE
卷 19, 期 2, 页码 263-268出版社
WILEY
DOI: 10.1002/pro.313
关键词
bovine insulin; oxidative damage; evaluation strategy; cyclic voltammetry
资金
- NSFC [20607011, 20875055]
- Ministry of Education of China [708058]
- Excellent Young Scientists and Key Science-Technology Project in Shandong Province [2007BS08005, 2008GG10006012]
- [NCET-06-0582]
Research on protein oxidative damage may give insight into the nature of protein functions and pathological conditions. In this work, the oxidative damage of bovine insulin on Au electrode was investigated by cyclic voltammetry (CV). The experimental results show that there are two anodic peaks for the oxidative damage of bovine insulin, which arise from the oxidation of the exposed disulfide bond SAS(CYS7A,CYS7B), forming sulfenic acid RSOH (1.20 V, vs. SCE), sulfinic acid RSO2H and sulfonic acid RSO3H (1.35 V, vs. SCE). These in vitro findings not only demonstrate the applicability of CV in simulating/evaluating the oxidative damage of nonredox proteins but also find two promising candidates (two anodic peaks) for measuring insulin.
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