PCRless library mutagenesis via oligonucleotide recombination in yeast

The directed evolution of biomolecules with new functions is largely performed in vitro, with PCR mutagenesis followed by high-throughput assays for desired activities As synthetic biology creates impetus for generating biomolecules that function in living cells, new technologies are needed for performing mutagenesis and selection for directed evolution in vivo Homologous recombination, routinely exploited for targeted gene alteration, is an attractive tool for in vivo library mutagenesis, yet surprisingly is not routinely used for this purpose Here, we report the design and characterization of a yeast-based system for library mutagenesis of protein loops via oligonucleotide recombination In this system, a linear vector is co-transformed with single-stranded mutagenic oligonucleotides Using repair of nonsense codons engineered in three different active-site loops in the selectable marker TRP1 as a model system, we first optimized the recombination efficiency Single-loop recombination was highly efficient, averaging 5%, or 4 0 X 10(5) recombinants Multiple loops could be simultaneously mutagenized, although the efficiencies dropped to 0 2%, or 6 0 x 10(3) recombinants, for two loops and 0 01% efficiency, or 1 5 x 10(2) recombinants, for three loops Finally, the utility of this system for directed evolution was tested explicitly by selecting functional variants from a mock library of 1 10(6) wild-type nonsense codons Sequencing showed that oligonucleotide recombination readily covered this large library, mutating not only the target codon but also encoded silent mutations on either side of the library cassette Together these results establish oligonucleotide recombination as a simple and powerful library mutagenesis technique and advance efforts to engineer the cell for fully in vivo directed evolution