期刊
PROTEIN SCIENCE
卷 18, 期 5, 页码 960-969出版社
WILEY
DOI: 10.1002/pro.106
关键词
self-assembly; nanofilament; protein design; molecular modeling; protein nanomaterial
Self-assembly of artificially designed proteins is extremely desirable for nanomaterials. Here we show a novel strategy for the creation of self-assembling proteins, named Nanolego.'' Nanolego consists of structural elements'' of a structurally stable symmetrical homo-oligomeric protein and binding elements,'' which are multiple heterointeraction proteins with relatively weak affinity. We have established two key technologies for Nanolego, a stabilization method and a method for terminating the self-assembly process. The stabilization method is mediated by disulfide bonds between Cysteine-residues incorporated into the binding elements, and the termination method uses capping Nanolegos,'' in which some of the binding elements in the Nanolego are absent for the self-assembled ends. With these technologies, we successfully constructed timing-controlled and size-regulated filament-shape complexes via Nanolego self-assembly. The Nanolego concept and these technologies should pave the way for regulated nanoarchitecture using designed proteins.
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