期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 92, 期 1, 页码 77-87出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2013.07.017
关键词
N-terminus modifications; Cytochrome P450s; Cytochrome P450 protein; Recombinant expression; Expression systems; Silent mutations
类别
资金
- American Heart Association
- University of Illinois Urbana Champaign School of Molecular and Cellular Biology
- University of Illinois Urbana-Champaign
- University of Illinois Urbana-Champaign Research Board
Recombinant expression of membrane-bound cytochrome P450s in bacterial expression systems provide a well-established system capable of producing large yields of catalytically active protein. As the biochemical knowledge regarding cytochrome P450s increases, so does the efficiency of protein expression through various modifications that do not disrupt the functional properties of the protein of interest. Changes such as N-terminal modifications, reduction of secondary mRNA structure, bacterial codon usage, selection of vector and host strain, as well as varying external growth conditions all appear to influence protein expression. Several optimizations are often required for sufficient expression of cytochrome P450s at the desired cellular localization. This review aims to comprehensively summarize and update the significant advances made in membrane protein P450 expression in bacterial expression systems. Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据