期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 83, 期 1, 页码 37-46出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2012.02.013
关键词
Heterologous protein expression; Bioengineering; Synthetic biology; Gene optimization; Codon usage
类别
资金
- NSF SBIR [0750206]
- NIH Roadmap Center [P50 GM073210]
- DNA2.0
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [0750206] Funding Source: National Science Foundation
The DNA sequence used to encode a polypeptide can have dramatic effects on its expression. Lack of readily available tools has until recently inhibited meaningful experimental investigation of this phenomenon. Advances in synthetic biology and the application of modern engineering approaches now provide the tools for systematic analysis of the sequence variables affecting heterologous expression of recombinant proteins. We here discuss how these new tools are being applied and how they circumvent the constraints of previous approaches, highlighting some of the surprising and promising results emerging from the developing field of gene engineering. (C) 2012 Elsevier Inc. All rights reserved.
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