期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 86, 期 1, 页码 27-34出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2012.08.014
关键词
Selenoprotein K; Selenoprotein S; Endoplasmic reticulum associated protein degradation; ERAD; Selenoproteins; Selenocysteine; SelK; SeIS; VIMP; Oxidative stress
类别
资金
- MRI-R2 Grant [0959496]
- National Center for Research Resources [5P30RR031160-03]
- Institute of General Medical Sciences [8 P30 GM103519-03]
- National Science Foundation [MCB-1054447]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1054447] Funding Source: National Science Foundation
Selenoprotein K (SelK) is a membrane protein residing in the endoplasmic reticulum. The function of SelK is mostly unknown: however, it has been shown to participate in anti-oxidant defense, calcium regulation and in the endoplasmic reticulum associated protein degradation (ERAD) pathway. In order to study the function of SelK and the role of selenocysteine in catalysis, we have tested heterologous expression of human SelK in E. coli. Consequently, we have developed an over-expression strategy that exploits the maltose binding protein as a fusion partner to stabilize and solubilize SelK. The fusion partner can be cleaved from SelK in the presence of a variety of detergents compatible with structural characterization and the protein purified to homogeneity. SelK acquires a helical secondary structure in detergent micelles, even though it was predicted to be an intrinsically disordered protein due to its high percentage of polar residues. The same strategy was successfully applied to preparation of SelK binding partner selenoprotein S (SelS). Hence, this heterologous expression and purification strategy can be applied to other members of the membrane enzyme family to which SelK belongs. (c) 2012 Elsevier Inc. All rights reserved.
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