期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 77, 期 2, 页码 153-158出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2011.01.006
关键词
Thermostable endoglucanase; Synthetic gene; Methylumbelliferyl-beta-D-cellobioside; Codon optimization; alpha-Mating factor signal peptide; Michaelis-Menten kinetic parameters
类别
资金
- Chevron Technology Ventures, Inc RSO [23]
- National Science Foundation (DGE) [0653984]
- Direct For Education and Human Resources
- Division Of Graduate Education [0653984] Funding Source: National Science Foundation
The endoglucanase (E1) from Acidothermus cellutolyticus has been used extensively in cellulase research. The goal of this work was to produce high levels of this enzyme in a system that facilitates purification. A codon-optimized synthetic gene for A. cellulolyticus E1 with a C-terminal histidine tag was cloned into the genome of Pichia pastoris. Strain KM71H expressed the most enzyme, with a yield of 550 mg/L culture supernatant. The temperature optimum (80 degrees C) and pH optimum (5.1) of the purified enzyme agree with previously determined values for the enzyme produced in other systems. Michaelis-Menten kinetic parameters were determined, using a fluorescent substrate (methylumbelliferyl-beta-D-cellobioside) at various temperatures. This thermostable enzyme can be used in future cellulosic biofuels-related research. (c) 2011 Elsevier Inc. All rights reserved.
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