期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 71, 期 1, 页码 91-95出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2009.12.009
关键词
Affinity purification; Affinity tag; Disordered protein; Silanol group; Silica-binding protein; Si-tag
类别
资金
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Japan Society for the Promotion of Science [21780096]
- New Energy and Industrial Technology Development Organization (NEDO) of Japan [09C46130a]
- Grants-in-Aid for Scientific Research [21780096] Funding Source: KAKEN
We previously reported that a silica-binding protein, designated Si-tag, can be used as a fusion tag to immobilize functional proteins on silica surfaces. In this study, by taking advantage of the strong affinity of Si-tag for silica, we developed a single-step purification method for Si-tagged fusion proteins. We utilized unmodified bare silica particles as a specific adsorbent and a high concentration of MgCl2 solution as an elution buffer. A fusion protein of Si-tag and immunoglobulin-binding staphylococcal protein A, designated Si-tagged protein A, was recovered with a purity of 87 +/- 3% and yield of 84 +/- 4% from a crude extract of recombinant Escherichia coli. The simplicity Of Our method enables rapid, cost-effective purification of Si-tagged fusion proteins. We also discuss the mechanism of binding and dissociation of Si-tag and silica surfaces, and we suggest that the unusual basicity and disordered Structure of the Si-tag polypeptide play important roles in the binding to silica. (C) 2009 Elsevier Inc. All rights reserved.
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