期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 60, 期 2, 页码 147-150出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2008.04.009
关键词
dynamic binding capacity; chromatography; protein purification; biolayer interferometry; light diffraction; protein assay
Biolayer interferometry is a novel method for quantifying macromolecules, such as proteins, in solution. The presence of other, non-binding molecules does not interfere with quantification, which allows one to measure the concentration of the molecule of interest in a crude mixture. Here we apply this method to determining the dynamic binding capacity of affinity resins. (c) 2008 Elsevier Inc. All rights reserved.
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