期刊
PROTEIN AND PEPTIDE LETTERS
卷 17, 期 11, 页码 1351-1362出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/0929866511009011351
关键词
Amphipathic; peptide; antimicrobial; membrane protein; linear dichroism; liposome; dynamic light scattering
Here we present data on the kinetics of insertion of melittin, a peptide from bee venom, into lipid membranes of different composition. Another component of bee venom is the enzyme phospholipase A2 (PLA(2)). We have examined the interaction of melittin and PLA(2) with liposomes both separately and combined and demonstrate that they work synergistically to disrupt the membranes. A dramatic difference in the action of melittin and PLA(2) is observed when the composition of the membrane is altered. Temperature also has a large effect on the kinetics of insertion and membrane disruption. We use a combination of techniques to measure liposome size (dynamic light scattering), peptide secondary structure (circular dichroism spectroscopy), peptide orientation relative to the membrane (linear dichroism spectroscopy) and enzymatic digestion of the lipids (mass spectrometry).
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