期刊
PROTEIN AND PEPTIDE LETTERS
卷 17, 期 5, 页码 585-590出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/092986610791112729
关键词
Ribosome display; In vitro selection; Antibody engineering; PlGF; scFv; Ovarian cancer
资金
- National Science Foundation of China [30672232]
- Science and Technology Committee of Shanghai [074119647]
- Health Bureau of Shanghai [2007147]
Ribosome display is a powerful cell-free technology to select a desired antibody together with its encoding mRNA. In this study, a human single-chain variable fragment (scFv) library was generated from the peripheral blood lymphocyte RNA of 10 ovarian cancer patients and then panned against bead-conjugated human PlGF, a protein that may contribute to the growth and metastasis of ovarian cancer. A selected scFv antibody was evaluated by Western blot and its affinity constant to the PlGF was determined by noncompetitive enzyme immunoassay. This study highlights the ribosomal display technology for the selection of human antibody from patient-derived gene pools.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据