期刊
PROSTATE
卷 74, 期 13, 页码 1297-1307出版社
WILEY
DOI: 10.1002/pros.22846
关键词
biophysical biomarkers; single-cell analysis; elasticity; adhesion; deformability
资金
- Integrative Cancer Biology Program [U54CA113001]
- Early Detection Research Network [U01CA086402]
- Cancer Center Support Grant [P30CA054174]
- National Institutes of Health
- University of Texas STARS Award
- Cancer Therapy and Research Center Fund
- Voelcker Fund
- GMaP Region 4 NCI Early Career Cancer Health Disparities Researcher Scholarship
BACKGROUND. Emerging evidence shows that nanomechanical phenotypes of circulating tumor cells (CTC) could become potential biomarkers for metastatic castration resistant prostate cancer (mCRPC). METHODS. To determine the nanomechanical phenotypes of CTCs we applied atomic force microscopy (AFM) employing the PeakForce quantitative nanomechanical (QNM) imaging. We assessed biophysical parameters (elasticity, deformation, and adhesion) of 130 CTCs isolated from blood samples from five castration sensitive (CS) and 12 castration resistant prostate cancer (CRPCa) patients. RESULTS. We found that CTCs from CRPCa patients are three times softer, three times more deformable, and seven times more adhesive than counterparts from CSPCa patients. Both nonsupervised hierarchical clustering and principle component analysis show that three combined nanomechanical parameters could constitute a valuable set to distinguish between CSPCa and CRPCa. CONLUSIONS. Our study indicates that nanomechanical phenotypes of CTCs may serve as novel and effective biomarkers for mCRPC. (C) 2014 Wiley Periodicals, Inc.
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