期刊
PROSTAGLANDINS & OTHER LIPID MEDIATORS
卷 109, 期 -, 页码 23-31出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.prostaglandins.2014.03.001
关键词
Oxylipins; Eicosanoids; Arachidonic acid; Eicosapentaenoic acid
资金
- Marie Curie Career Integration Grant
- US National Institutes of Health (NIH), Enviromental Health (NIEHS) [P42 ES004699, R01 ES002710]
- Diabetes and Digestive and Kidney Diseases (NIDDK) [U24 DK097154]
- West Coast Central Comprehensive Metabolomics Resource Core [WC3MRC]
Introduction: Several supplementation studies with long-chain omega-3 polyunsaturated fatty acids (LC n-3 PUFA) describe an increase of EPA-derived hydroxy, epoxy and dihydroxy fatty acids in blood, while changes in levels of other LC n-3 and n-6 PUFA-derived oxylipins were minor. In order to investigate the kinetics of changes in oxylipin levels in response to LC n-3 PUFA ingestion, we conducted a single dose treatment study with healthy subjects. Subjects and methods: In the present kinetic study, we compared patterns of hydroxy, epoxy and dihydroxy fatty acids in plasma of 6 healthy men before and after 6, 8, 24, and 48 h of fish oil (1008 mg EPA and 672 mg DHA) ingestion. Levels of EPA- as well as other LC PUFA-derived hydroxy, epoxy and dihydroxy fatty acids were analyzed in plasma by LC-MS. Additionally, levels of these oxylipins were compared with their parent PUFA levels in plasma phospholipids. Results: All EPA-derived oxylipin levels were significantly increased 6 h after LC n-3 PUFA ingestion and gradually drop thereafter reaching the baseline levels about 48 h after treatment. The relative increase in EPA plasma phospholipid levels highly correlated with the increase of plasma EPA-derived oxylipin levels at different time points. In contrast, plasma levels of arachidonic acid- and DHA-derived oxylipins as well as parent PUFA levels in plasma phospholipids were hardly changed. Discussion and conclusions: Our findings demonstrate that a single dose of LC n-3 PUFAs can rapidly induce a shift in the EPA oxylipin profile of healthy subjects within a few hours. Taking the high biological activity of the EPA-derived epoxy fatty acids into account, even short-term treatment with LC n-3 PUFAs may cause systemic effects, which warrant further investigation. (C) 2014 Elsevier Inc. All rights reserved.
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