期刊
ACS CATALYSIS
卷 5, 期 3, 页码 1604-1608出版社
AMER CHEMICAL SOC
DOI: 10.1021/cs502064s
关键词
artificial metabolism; multistep catalysis; synthetic biology; systems biocatalysis; futile cycle; functional group interconversion
资金
- SysBiocat COST Action [CM1303]
An in vitro artificial cycle interconverting chemical functional groups through a series of six enzyme-catalyzed reactions was set up. The addition of any of the substrates established a steady state in which concentrations of all the four components remained unchanged, and transformation of any component into another is possible by breaking the cycle omitting one (or more) of the enzymatic activities. We believe that this example of an artificial metabolism may constitute a novel approach toward the synthesis of useful products by modern applied biocatalysis. Such an in vitro strategy circumvents the presence of deviating/competing metabolic pathways as well as the issues related to enzymes inhibition or regulation as observed at the cellular level.
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