期刊
PROCESS BIOCHEMISTRY
卷 75, 期 -, 页码 1-9出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2018.09.002
关键词
Agro-industrial by-product; Submerged cultivation; Bioconversion; Antioxidant capacity; Antihypertensive activity; Antidiabetic activity
资金
- Conselho Nacional de Desenvolvimento Cientffico e Tecnologico - CNPq [402631/2016-1]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES
- CNPq
Feathers are recalcitrant protein-rich by-products generated by the poultry agro-industry, and valorization of residual biomasses from industrial processing is increasingly focused. Three feather-degrading Bacillus strains (CL18, CL33A, CL14) were used to convert feathers into feather hydrolysates (FH) during submerged cultivations, and the in vitro bioactivities of FHs were assessed. In feather broth (10 g/L feathers), Bacillus sp. CL18 degraded feathers more efficiently and the resulting FHs displayed enhanced antioxidant activities. With increasing feather concentrations, soluble protein was higher (9.02 mg/mL) after five days of cultivation with Bacillus sp. CL18 on feather broth containing 40 g/L feathers. This FH displayed antioxidant activities, as evaluated through the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical-scavenging assays (34.7% and 80.3%, respectively), Fe2+-chelating ability (80.6%), and reducing power (0.518 Abs(700)). In vitro antihypertensive and antidiabetic activities of FH were demonstrated through inhibition of angiotensin I-converting enzyme (ACE; 89.7%) and dipeptidyl peptidase-IV (DPP IV; 95.7%) activities, respectively. Half-maximal effective/inhibitory concentrations of FH were 5.39, 15.12, 10.50, 1.61 and 1.52 mg/mL in the ARTS, DPPH, Fe2+-chelating, ACE- and DPP IV-inhibitory assays, respectively. Antioxidant, antidiabetic and antihypertensive potentials of FHs indicate microbial conversion as a suitable ecofriendly technology for feathers reclamation and valorization within the biorefinery concept.
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