Functional cello-oligosaccharides production from the corncob residues of xylo-oligosaccharides manufacture

An integrated process has been developed, consisting of the adsorption-separation of cellulase enzymes to selectively remove beta-glucosidase, and multi-stage enzymatic hydrolysis of corncob residues from xylo-oligosaccharides manufacture with the beta-glucosidase deficient cellulase, aiming to obtain a high yield of cello-oligosaccharides production. After the adsorption-separation process, 79.50% of the endoglucanase was retained in substrate, whereas 90.67% of beta-glucosidase was removed with the separated liquid fraction, utilizing the different adsorbability of these enzymes to the substrate. A three-stage enzymatic hydrolysis of corncob residues with the beta-glucosidase deficient cellulase was proposed in which the first, the second and the third stage were conducted for 6, 6h and 12h, respectively. Analysis indicated that the removal of hydrolysis products (glucose and cello-oligosaccharides) at each stage improved cello-oligosaccharides productivity and enzymatic hydrolysis yield. The cello-oligosaccharides yield and enzymatic hydrolysis yield in three-stage enzymatic hydrolysis were significantly improved to 51.78% and 75.56%, respectively, which were 36.00% and 25.10% higher than single-stage hydrolysis with original cellulase enzymes. (C) 2014 Elsevier Ltd. All rights reserved.