期刊
PROCESS BIOCHEMISTRY
卷 49, 期 10, 页码 1757-1766出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2014.06.022
关键词
Phycobiliproteins; Lyngbya sp A09DM; Triton X-100; Antioxidant activity, Caenorhabditis elegans; Anti-aging activity
资金
- Department of Science and Technology (DST), New Delhi, India [IF120712]
- Department of Biotechnology (DBT), New Delhi, India [BT/01 /CEIB/09/V/05]
The present study probes into the purification of phycobiliproteins, and characterization of their in vitro anti-oxidant activity. Moreover, the study also demonstrates the use of antioxidant virtue of phycoerythrin in moderating the phenomenon of aging in Caenorhabditis elegans. Phycoerythrin, phycocyanin and allophycocyanin were purified successfully from Lyngbya sp. A09DM by ammonium sulfate fractionation appended with Triton X-100 intercession. The success of protocol was examined by a series of biochemical characterization like SDS-PAGE, native-PAGE, UV-visible spectroscopy and fluorescence spectroscopy ensuring purity, integrity and functionality of purified phycoerythrin, phycocyanin and allophycocyanin. Purified phycobiliproteins were evaluated for antioxidant and metal ion chelating activity by various in vitro antioxidant assay systems. Results showed significant and dose-dependent antioxidant as well as metal chelating potential of all phycobiliproteins in decreasing order of phycoerythrin > phycocyanin > allophycocyanin. Expansion in lifespan and improvement in pharyngeal pumping of C. elegans were noticed upon pre-treatment with phycoerythrin (100 mu g ml(-1)). Moreover, phycoerythrin mediated increase in worm survival under oxidative stress revealed that the life expansion effect of phycoerythrin on nematode is in part by an action of its antioxidant virtue. These results collectively added up evidence in favor of the 'free-radical theory of aging'. The present report, for the first time, describes antioxidant potential of phycoerythrin and its use in extending life-span of C. elegans. (C) 2014 Elsevier Ltd. All rights reserved.
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