期刊
PROCESS BIOCHEMISTRY
卷 48, 期 3, 页码 426-429出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.02.014
关键词
Hydroxydaidzein; Daidzein; Aspergillus oryzae; Cytochrome P450 monooxygenase; Pichia pastoris
CYP57B3 from Aspergillus oryzae was recently discovered to catalyze the ortho-hydroxylation of the soyisoflavone genistein. In the present study, the gene encoding CYP57B3 was fused with the reductase domain of the CYP102A1 gene (BM3R) from Bacillus megaterium, and recombinant Pichia pastoris harboring the P450 fusion gene was evaluated for its ability to produce ortho-hydroxydaidzein derivatives from daidzein. The results showed that 8-hydroxydaidzein (8-OHDe), 3'-hydroxydaidzein (3'-OHDe), and 6-hydroxydaidzein (6-OHDe) were produced during fermentation with a maximal conversion of 2.4, 0.9, and 36.3%, respectively. The maximal yield of 6-OHDe by the recombinant strain was 9.1 mg/l. To our knowledge, both the maximal yield and the conversion efficiency of 6-OHDe from daidzein in the present. study are the highest among those reported in the literatures to date. The present study is also the first to demonstrate production of ortho-hydroxydaidzein derivatives using a fusion fungus cytochrome P450 enzyme. (C) 2013 Elsevier Ltd. All rights reserved.
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