期刊
PROCESS BIOCHEMISTRY
卷 48, 期 2, 页码 325-330出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2012.10.016
关键词
Ruditapes philippinarum; Enzymatic hydrolysis; Antioxidant peptide; Free radical
资金
- Konkuk University
We purified a novel antioxidant peptide from Ruditapes philippinarum (R. philippinarum) and investigated its free radical scavenging activities. To prepare the peptide, eight proteases were tested for enzymatic hydrolysis. alpha-chymotrypsin hydrolysate, which showed clearly superior hydroxyl radical scavenging activity (p < 0.05), were further purified using a flow filtration system and consecutive chromatographic methods. Finally, a novel antioxidant peptide was obtained, and the sequence was identified as Ser-Val-Glu-Ile-Gln-Ala-Leu-Cys-Asp-Met. The peptide from R. philippinarum effectively scavenged hydroxyl, DPPH, alkyl and superoxide radicals, with observed IC50 values of 0.042, 0.091, 0.107 and 0.372 mg/ml, respectively. This is the first report of an antioxidant peptide derived from the hydrolysates of R. philippinarum which, further, possesses competitive free radical quenching potential. (C) 2012 Elsevier Ltd. All rights reserved.
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