期刊
PROCESS BIOCHEMISTRY
卷 48, 期 9, 页码 1293-1302出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.06.019
关键词
Oil palm trunk (OPT); Cellulases; Xylanase; Solid-state fermentation; Saccharification
资金
- GUP Research grant by Universiti Teknologi Malaysia [Q.J130000.2535.00H88]
- MyBrain15 - Ministry of Higher Education Malaysia (MOHE)
Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1 x 10(8) spore/g (inoculum) with 125 mu m of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27,3.36, 4.54 and 418.70 U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), beta-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40 degrees C compared to their optimum activities conditions (60 degrees C for FPase and 70 degrees C for CMCase, beta-glucosidase and xylanase). SOS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and beta-glucosidase), xylanase and protease. Enzymatic degradation of alkaline treated OPT with concentrated crude cellulases and xylanases resulted in producing polyoses. (C) 2013 Elsevier Ltd. All rights reserved.
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