期刊
PROCESS BIOCHEMISTRY
卷 47, 期 6, 页码 901-907出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2011.06.012
关键词
Linum album; Piriformospora indica; Podophyllotoxin; 6-Methoxypodophyllotoxin; Phenylalanine ammonia lyase
资金
- Council of Scientific and Industrial Research, India
The effect of addition of autoclaved and filter-sterilized culture filtrate of Piriformospora indica (a root endophytic fungus) to the growing Linum album hairy root cultures on growth and lignan production was investigated. The addition resulted in a significant enhancement in lignan production and growth. The podophyllotoxin and 6-methoxypodophyllotoxin (the lignans) concentrations were maximally improved by 3.8 times (233.8 mg/L) and 4.4 times (131.9 mg/L) in comparison to control cultures, respectively, upon addition of 3.0% (v/v) filter-sterilized culture filtrate of P. indica to the hairy root cultures of L. album for exposure time 01 48 h. This increase in the lignan content also coincided with the increase in phenylalanine ammonia lyase activity, which was 3.1-fold (371.4 mu kat/kg protein) higher compared to control cultures under the same conditions. The maximal increase in hairy root biomass was, however, obtained under different conditions; it was enhanced by 1.4 times (21.8 g/L) in comparison to control cultures, when 2% (v/v) filter-sterilized culture filtrate was in contact with L. album cultures for 96 h. (C) 2011 Elsevier Ltd. All rights reserved.
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