期刊
PROCESS BIOCHEMISTRY
卷 47, 期 10, 页码 1516-1520出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2012.06.004
关键词
Antibacterial; Autoclaving; Gum olibanum; Silver nanoparticles; Surface-enhanced Raman scattering
A simple and ecofriendly biosynthetic process has been developed for silver nanoparticles using the aqueous extract of gum olibanum (Boswellia serrata), a renewable natural plant biopolymer. The water soluble compounds in the gum serve as dual functional reducing and stabilizing agents. The effect of concentration of gum and silver nitrate: and reaction time on nanoparticle synthesis was studied. The UV-visible spectroscopy, transmission electron microscopy and X-ray diffraction techniques were used to characterize the synthesized nanoparticles. By tuning the reaction conditions, size controlled spherical nanoparticles of around 7.5 +/- 3.8 nm was achieved. Using Fourier transform infrared spectroscopy and Raman spectroscopy, a probable mechanism involved in reduction and stabilization of nanoparticles has been explained. The produced silver nanoparticles exhibited substantial antibacterial activity on both the Gram classes of bacteria. By virtue of being biogenic and encapsulated with proteins, these surface functionalized nanoparticles can be easily integrated for various biological applications. (C) 2012 Elsevier Ltd. All rights reserved.
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