期刊
PROCESS BIOCHEMISTRY
卷 47, 期 6, 页码 1011-1015出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2012.03.019
关键词
Continuous fermentation; Saccharomyces cerevisiae; Pichia stipitis; Immobilized cell microchannel bioreactor; Catabolite repression
资金
- National Research Foundation of Korea (NRF) [2010-0027563]
- Korea government (MEST)
- Korea Institute of Energy Technology Evaluation and Planning (KETEP) [20114010203070]
- Korea government Ministry of Knowledge Economy
- Korea Evaluation Institute of Industrial Technology (KEIT) [20114010203070] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
An immobilized cell microchannel bioreactor was designed to test continuous fermentation. The fermentation set-up included a bottom hydrophilic quartz channel to immobilize cells using 0.4 wt% polyethyleneimine and a top channel designed to continuously remove metabolically generated carbon dioxide using hydrophobic polypropylene. To evaluate fermentation characteristics of immobilized cells, ethanol fermentation was carried out using Saccharomyces cerevisiae and Pichia stipitis. The immobilized cell microchannel bioreactor was used to identify long-term activity of immobilized S. cerevisiae cells. The continuous flow microchannel bioreactor was operated stably over a period of 1 month. The immobilized cell microchannel bioreactor was used to examine the characteristics cells that consumed mixed substrates. The concentration ratio of glucose to xylose for simultaneous utilization of hemicellulosic sugars was evaluated using the microchannel bioreactor and the results were compared with those obtained by using conventional batch fermentation with P. stipitis. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据