期刊
PROCESS BIOCHEMISTRY
卷 47, 期 3, 页码 533-537出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2011.11.017
关键词
Candida antarctica lipase A; Organic solvents; Catalytic activity; Secondary structure; Fourier transform-infrared spectroscopy
资金
- Guangdong Key Technology RD Program [2009802020-1007]
- Guangzhou Key Technology RD Program [2009A1-E021]
- Fundamental Research Funds for the Central Universities [2011ZZ0018]
The effect of various organic solvents on the hydrolytic and esterification activities of lipase A from Candida antarctica (CALA) was investigated. Compared to the control group, the esterification and hydrolytic activities of CALA both increased with treatment of acetonitrile or acetone. However, the catalytic activity decreased after treatment with other organic solvents, especially with ethanol or ethyl acetate. Moreover, with treatment by the same organic solvents, the residual esterification activity of CALA was much higher than the hydrolytic activity for the olive oil. Results of Fourier transform-infrared spectroscopy analysis implied that the catalytic activity variance was mainly attributed to the secondary structure changes of CALA. The acetonitrile treatment could also increase the thermal and pH stability of CALA. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.
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