期刊
PROCESS BIOCHEMISTRY
卷 45, 期 5, 页码 794-798出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2009.12.015
关键词
Achromobacter xylosoxidans; MTBE Biodegradation; Two-dimensional gel electrophoresis proteome
资金
- Spanish Ministerio de Medio Ambiente
The gasoline additive methyl tert-butyl ether (MTBE) can contaminate groundwater and soil. In order to eliminate it, several methods are being developed, among which bioremediation - that is, the addition of microbial cultures that can degrade the compound - holds promise. Our laboratory has identified Achromobacter xylosoxidans MCM1/1 as an MTBE-degrading bacterial strain. It degrades 78% of this chemical in 5 days. In this study we also analyze the effects of MTBE on the biology of A. xylosoxidans MCM1/1 and compare its proteomic profile after incubation with MTBE with that of unchallenged bacteria. The 2D proteomic analysis shows that the following four proteins are induced by MTBE: 50S ribosomal protein LW, amino acid-binding periplasmic protein, ATP synthase and endoribonuclease L Characterizing the bacterial response to MTBE at the biochemical level identifies proteins that can be used by biocatalysts for soil and water bioremediation. (C) 2010 Elsevier Ltd. All rights reserved.
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