期刊
PROCESS BIOCHEMISTRY
卷 45, 期 9, 页码 1504-1510出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2010.05.032
关键词
Pseudomonas aeruginosa; Rhamnolipid; Carbon and nitrogen source; Bioremediation; Proteomics
资金
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
- Petroleo Brasileiro S.A.
The PA1 strain of Pseudomonas aeruginosa isolated from oil waste produces rhamnolipid, a biodegradable surfactant with applications in several industrial and environmental fields. The metabolic pathway and genetic regulation of rhamnolipid production in P. aeruginosa are poorly understood. Herein, several proteins directly or indirectly related to rhamnolipid production and their genetic regulations were identified by comparative proteomic. We compared the proteome of P. aeruginosa PA1 after fermentation in two different conditions of carbon and nitrogen sources: condition A allowed rhamnolipid production and condition B prevented it. Protein extracts from cellular pellets were compared using 2D-PAGE stained with colloidal Coomassie followed by MALDI-TOF/TOF mass spectrometry. We identified 21 differentially expressed proteins, including those involved in secretion, quorum sensing, oxidative response and metabolism. (C) 2010 Elsevier Ltd. All rights reserved.
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