期刊
PROCESS BIOCHEMISTRY
卷 45, 期 1, 页码 67-74出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2009.08.004
关键词
Fed-batch culture; hCTLA4Ig; Transgenic plant cell culture; RAmy3D promoter; Oryza sativa
资金
- Ministry of Commerce, Industry, and Energy of the Republic of Korea [A18-014]
- Center for Advanced Bioseparation Technology, KOSEF
RAmy3D promoter is capable of expressing high levels of recombinant proteins in response to the depletion of sugar in transgenic rice cell suspension cultures. For this reason, it is necessary to change the growth medium into sugar-free production medium to produce the target protein, human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig), using the inducible RAmy3D promoter. Since the two-stage Culture is a complex process to perform in large-scale, a fed-batch method was evaluated with the addition of concentrated amino acids before the depletion of sugar to induce hCTLA4Ig production. This fed-batch culture was found to be effective and the production of hCTLA4Ig was enhanced up to 1.2-fold compared to that of two-stage cultures with medium exchange. In addition, when this fed-batch culture was performed in a 15-1 stirred-tank bioreactor, maximum hCTLA4Ig level was 76.5 mg l(-1) at day 10. (C) 2009 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据