期刊
PROCESS BIOCHEMISTRY
卷 45, 期 4, 页码 613-616出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2009.12.009
关键词
2,3-butanediol; Corncob; Detoxification; Fermentation; Hydrolysate; Klebsiella oxytoca
资金
- '863' Hi-Tech Research and Development Program of China [2007AA100702-3]
- Shanxi Key Project [20090322025]
Corncob acid hydrolysate, detoxed by sequently boiling, overliming and activated charcoal adsorption, was used for 2,3-butanediol production by Klebsiella oxytoca ACCC 10370. The effects of acetate in hydrolysate and pH on 2,3-butanediol production were investigated. It was found that acetic acid in hydrolysate inhibited the growth of K. oxytoca while benefited the 2,3-butanediol yield. With the increase in acetic acid concentration in medium from 0 to 4 g/l, the lag phase was prolonged and the specific growth rate decreased. The acetic acid inhibition on cell growth can be alleviated by adjusting pH to 6.3 prior to fermentation and a substrate fed-batch strategy with a low initial acetic acid concentration. Under the optimum condition, a maximal 2,3-butanediol concentration of 35.7 g/l was obtained after 60 h of fed-batch fermentation, giving a yield of 0.5 g/g reducing sugar and a productivity of 0.59 g/h l. (C) 2009 Elsevier Ltd. All rights reserved.
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