期刊
PROCESS BIOCHEMISTRY
卷 44, 期 8, 页码 886-890出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2009.04.012
关键词
2-Phenylethanol; Flavor; Biotransformation; Saccharomyces cerevisiae; In situ product adsorption; Macroporous resin
资金
- Foundation of Zhejiang Key Developing Discipline of Pharmacy, Zhejiang province, P.R. China
- State Natural Science Fund [30570053]
An in situ product adsorption technique was used to enhance the biotransformation of L-phenylalanine to 2-phenylethanol by Saccharomyces cerevisiae BID. As a suitable adsorbent, the non-polar macroporous resin D101, selected from several resins tested, showed high adsorption capacity for 2-phenylethanol but not L-phenylalanine. Product inhibition was effectively alleviated by the addition of macroporous resin D101 to the biotransformation medium. When 2 g of hydrated resin D101 was added to 30 mL of the biotransformation medium, the total 2-phenylethanol concentration achieved was 6.17 g/L, of which 3.15 g/L remained in the aqueous phase and 3.02 g/L was adsorbed onto the resin. The molar yield of 2-phenylethanol reached 0.70 after 24 h cultivation. Addition of the macroporous resin greatly increased the volumetric productivity of 2-phenylethanol, and made the downstream processing more feasible and easier to perform in an industrial application. (C) 2009 Elsevier Ltd. All rights reserved.
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