期刊
PROCESS BIOCHEMISTRY
卷 43, 期 7, 页码 743-747出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2008.02.019
关键词
ACE-inhibitory peptide; hard clam; protein hydrolysate; angiotensin I converting enzyme; inhibition type; gel filtration
The meat of hard clam was extracted using hot water. The residual meat was freeze-dried then hydrolyzed at 50 degrees C for 5 h by Protamex (PX). The inhibitory effects of hot water extract and hydrolysate against angiotensin I converting enzyme (ACE) were investigated. The IC(50) value of hot water extract and hydrolysate on ACE were 1.090 and 0.036 mg/ml, respectively. The PX hydrolysate was separated into five fractions by size exclusion chromatography on a Sephadex G-25 column. The fifth fraction of the hydrolysate having molecular weight ranged 350-300 Da showed the highest inhibitory efficiency ratio (IER) being 5831%/(mg ml). The amino acid sequence of the inhibitory peptide was Tyr-Asn (IC(50) = 51 mu M). The hydrolysate showed mixed-type inhibition kinetics while Captopril, the positive control, showed competitive inhibition on ACE. Their K(i) values were 0.027 mg/ml and 0.0067 mu g/ml, respectively. (C) 2008 Elsevier Ltd. All rights reserved.
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