期刊
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
卷 280, 期 1759, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rspb.2012.2987
关键词
melanopsin; action spectrum; photopigment; G-protein; intrinsically photosensitive retinal ganglion cells
资金
- Medical Research Council
- BBSRC
- BBSRC [BB/K002252/1] Funding Source: UKRI
- MRC [G0801731] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/K002252/1] Funding Source: researchfish
- Medical Research Council [G0801731] Funding Source: researchfish
A subset of mammalian retinal ganglion cells expresses an opsin photopigment (melanopsin, Opn4) and is intrinsically photosensitive. The human retina contains melanopsin, but the literature lacks a direct investigation of its spectral sensitivity or G-protein selectivity. Here, we address this deficit by studying physiological responses driven by human melanopsin under heterologous expression in HEK293 cells. Luminescent reporters for common second messenger systems revealed that light induces a high amplitude increase in intracellular calcium and a modest reduction in cAMP in cells expressing human melanopsin, implying that this pigment is able to drive responses via both G(q) and G(i/o) class G-proteins. Melanopsins from mouse and amphioxus had a similar profile of G-protein coupling in HEK293 cells, but chicken Opn4m and Opn4x pigments exhibited some G(s) activity in addition to a strong G(q/11) response. An action spectrum for the calcium response in cells expressing human melanopsin had the predicted form for an opsin : vitamin A1 pigment and peaked at 479 nm. The G-protein selectivity and spectral sensitivity of human melanopsin is similar to that previously described for rodents, supporting the utility of such laboratory animals for developing methods of manipulating this system using light or pharmacological agents.
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