期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 115, 期 37, 页码 E8783-E8792出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1807501115
关键词
sorghum; stem; pith parenchyma; programmed cell death; NAC transcription factor
资金
- Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics for Agricultural Innovation) [QTL5503, QTL5506]
- JST [Core Research for Evolutional Science and Technology (CREST)] [JPMJCR12B5]
- JST [PRESTO] [JPMJPR11B3]
- Ministry of Education, Culture, Sports, Science and Technology of Japan KAKENHI [16H01247]
- Japan Society for the Promotion of Science KAKENHI [16H06172, 17H05019]
- Grants-in-Aid for Scientific Research [16H01247, 16H06172, 17H05019] Funding Source: KAKEN
Pith parenchyma cells store water in various plant organs. These cells are especially important for producing sugar and ethanol from the sugar juice of grass stems. In many plants, the death of pith parenchyma cells reduces their stem water content. Previous studies proposed that a hypothetical D gene might be responsible for the death of stem pith parenchyma cells in Sorghum bicolor, a promising energy grass, although its identity and molecular function are unknown. Here, we identify the D gene and note that it is located on chromosome 6 in agreement with previous predictions. Sorghum varieties with a functional D allele had stems enriched with dry, dead pith parenchyma cells, whereas those with each of six independent nonfunctional D alleles had stems enriched with juicy, living pith parenchyma cells. D expression was spatiotemporally coupled with the appearance of dead, air-filled pith parenchyma cells in sorghum stems. Among D homologs that are present in flowering plants, Arabidopsis ANAC074 also is required for the death of stem pith parenchyma cells. D and ANAC074 encode previously uncharacterized NAC transcription factors and are sufficient to ectopically induce programmed death of Arabidopsis culture cells via the activation of autolytic enzymes. Taken together, these results indicate that D and its Arabidopsis ortholog, ANAC074, are master transcriptional switches that induce programmed death of stem pith parenchyma cells. Thus, targeting the D gene will provide an approach to breeding crops for sugar and ethanol production.
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