期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 115, 期 34, 页码 8517-8522出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1807562115
关键词
cable bacteria; atomic force microscopy; dissection; interior structure
资金
- Danish National Research Foundation
- Aarhus Universitets Forskningsfond (AUFF NOVA-project)
- Villum Foundation
- Lundbeck Foundation
- EU H2020 RISE 2016 [MNR4SCell 734174]
- National Program for Support of Top-notch Young Professionals
- China Aerospace Science and Technology Corporation-Harbin Institute of Technology Joint Center for Technology Innovation Fund [HIT15-1A01]
- Harbin city science and technology projects [2013DB4BP031, RC2014QN017035]
- Materials Synthesis and Simulation Across Scales Initiative through Laboratory Directed Research and Development program at Pacific Northwest National Laboratory
- Department of Energy by Battelle [DE-AC05-76RL01830]
- National Science Foundation of China [21605102]
- Thousand Youth Talents Plan
- Carlsberg Foundation Internationalization fellowships [CF16-0757]
- Danish Council for Independent Research/Technology and Production
Filamentous Desulfobulbaceae bacteria were recently discovered as long-range transporters of electrons from sulfide to oxygen in marine sediments. The long-range electron transfer through these cable bacteria has created considerable interests, but it has also raised many questions, such as what structural basis will be required to enable micrometer-sized cells to build into centimeter-long continuous filaments? Here we dissected cable bacteria cells in vitro by atomic force microscopy and further explored the interior, which is normally hidden behind the outer membrane. Using nanoscale topographical and mechanical maps, different types of bacterial cell-cell junctions and strings along the cable length were identified. More important, these strings were found to be continuous along the bacterial cells passing through the cell-cell junctions. This indicates that the strings serve an important function in maintaining integrity of individual cable bacteria cells as a united filament. Furthermore, ridges in the outer membrane are found to envelop the individual strings at cell-cell junctions, and they are proposed to strengthen the junctions. Finally, we propose a model for the division and growth of the cable bacteria, which illustrate the possible structural requirements for the formation of centimeter-length filaments in the recently discovered cable bacteria.
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