期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 35, 页码 12883-12888出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1410551111
关键词
extracellular electron transfer; bioelectronics; respiration; membrane cytochromes
资金
- Air Force Office of Scientific Research Young Investigator Research Program Grant [FA9550-10-1-0144]
- Division of Chemical Sciences, Geosciences, and Biosciences, Office of Basic Energy Sciences of the US Department of Energy Grant [EF-1104831]
- Shewanella Federation consortium - Genomics: Genomes to Life program of the US Department of Energy Office of Biological and Environmental Research
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1359578] Funding Source: National Science Foundation
- Emerging Frontiers
- Direct For Biological Sciences [1104834] Funding Source: National Science Foundation
Bacterial nanowires offer an extracellular electron transport (EET) pathway for linking the respiratory chain of bacteria to external surfaces, including oxidized metals in the environment and engineered electrodes in renewable energy devices. Despite the global, environmental, and technological consequences of this biotic-abiotic interaction, the composition, physiological relevance, and electron transport mechanisms of bacterial nanowires remain unclear. We report, to our knowledge, the first in vivo observations of the formation and respiratory impact of nanowires in the model metal-reducing microbe Shewanella oneidensis MR-1. Live fluorescence measurements, immunolabeling, and quantitative gene expression analysis point to S. oneidensis MR-1 nanowires as extensions of the outer membrane and periplasm that include the multiheme cytochromes responsible for EET, rather than pilin-based structures as previously thought. These membrane extensions are associated with outer membrane vesicles, structures ubiquitous in Gram-negative bacteria, and are consistent with bacterial nanowires that mediate long-range EET by the previously proposed multistep redox hopping mechanism. Redox-functionalized membrane and vesicular extensions may represent a general microbial strategy for electron transport and energy distribution.
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