期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 23, 页码 8518-8523出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1324212111
关键词
retinal ganglion cells; rods; cones
资金
- Institut National de la Sante et de la Recherche Medicale, Universite Pierre-et-Marie-Curie-Universite Paris
- Agence Nationale de la Recherche (ANR) [GPiPS: ANR-2010-RFCS005]
- French state funds [ANR-11-IDEX-0004-02, ANR-10-LABX-65]
- Association Francaise contre les Myopathies-Telethon
- Regional Council of Ile-de-France
Progress in retinal-cell therapy derived from human pluripotent stem cells currently faces technical challenges that require the development of easy and standardized protocols. Here, we developed a simple retinal differentiation method, based on confluent human induced pluripotent stem cells (hiPSC), bypassing embryoid body formation and the use of exogenous molecules, coating, or Matrigel. In 2 wk, we generated both retinal pigmented epithelial cells and self-forming neural retina (NR)-like structures containing retinal progenitor cells (RPCs). We report sequential differentiation from RPCs to the seven neuroretinal cell types in maturated NR-like structures as floating cultures, thereby revealing the multipotency of RPCs generated from integration-free hiPSCs. Furthermore, Notch pathway inhibition boosted the generation of photoreceptor precursor cells, crucial in establishing cell therapy strategies. This innovative process proposed here provides a readily efficient and scalable approach to produce retinal cells for regenerative medicine and for drug-screening purposes, as well as an in vitro model of human retinal development and disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据