期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 34, 页码 12492-12497出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1323955111
关键词
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资金
- March of Dimes [21-FY07-610]
- Talpiot Medical Leadership Program Award
- Sheba Medical Center
- Sheba RD Grant
Oocyte endowment dwindles away during prepubertal and adult life until menopause occurs, and apoptosis has been identified as a central mechanism responsible for oocyte elimination. A few recent reports suggest that uncontrolled inflammation may adversely affect ovarian reserve. We tested the possible role of the proinflammatory cytokine IL-1 in the age-related exhaustion of ovarian reserve using IL-1 alpha and IL-1 beta-KO mice. IL-1 alpha-KO mice showed a substantially higher pregnancy rate and litter size compared with WT mice at advanced age. The number of secondary and antral follicles was significantly higher in 2.5-mo-old IL-1 alpha-KO ovaries compared with WT ovaries. Serum anti-Mullerian hormone, a putative marker of ovarian reserve, was markedly higher in IL-1 alpha-KO mice from 2.5 mo onward, along with a greater ovarian response to gonadotropins. IL-1 beta-KO mice displayed a comparable but more subtle prolongation of ovarian lifespan compared with IL-1 alpha-KO mice. The protein and mRNA of both IL-1 alpha and IL-1 beta mice were localized within the developing follicles (oocytes and granulosa cells), and their ovarian mRNA levels increased with age. Molecular analysis revealed decreased apoptotic signaling [higher B-cell lymphoma 2 (BCL-2) and lower BCL-2-associated X protein levels], along with a marked attenuation in the expression of genes coding for the proinflammatory cytokines IL-1 beta, IL-6, and TNF-alpha in ovaries of IL-1 alpha-KO mice compared with WT mice. Taken together, IL-1 emerges as an important participant in the age-related exhaustion of ovarian reserve in mice, possibly by enhancing the expression of inflammatory genes and promoting apoptotic pathways.
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