期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 29, 页码 10732-10737出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1321785111
关键词
amyloid; Parkinson disease
资金
- Ellison Medical Foundation
- Wilder Family Fellowship
- Santa Fe HealthCare Alzheimer's Disease Research Center
- Michael J. Fox Foundation
- National Parkinson Foundation
- University of Florida
It has been hypothesized that alpha-synuclein (alpha S) misfolding may begin in peripheral nerves and spread to the central nervous system (CNS), leading to Parkinson disease and related disorders. Although recent data suggest that alpha S pathology can spread within the mouse brain, there is no direct evidence for spread of disease from a peripheral site. In the present study, we show that hind limb intramuscular (IM) injection of alpha S can induce pathology in the CNS in the human Ala53Thr (M83) and wild-type (M20) alpha S transgenic (Tg) mouse models. Within 2-3 mo after IM injection in alpha S homozygous M83 Tg mice and 3-4 mo for hemizygous M83 Tg mice, these animals developed a rapid, synchronized, and predictable induction of widespread CNS alpha S inclusion pathology, accompanied by astrogliosis, microgliosis, and debilitating motor impairments. In M20 Tg mice, starting at 4 mo after IM injection, we observed alpha S inclusion pathology in the spinal cord, but motor function remained intact. Transection of the sciatic nerve in the M83 Tg mice significantly delayed the appearance of CNS pathology and motor symptoms, demonstrating the involvement of retrograde transport in inducing alpha S CNS inclusion pathology. Outside of scrapie-mediated prion disease, to our knowledge, this findiing is the first evidence that an entire neurodegenerative proteinopathy associated with a robust, lethal motor phenotype can be initiated by peripheral inoculation with a pathogenic protein. Furthermore, this facile, synchronized rapid-onset model of alpha-synucleinopathy will be highly valuable in testing disease-modifying therapies and dissecting the mechanism(s) that drive alpha S-induced neurodegeneration.
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